please see this forum conversation for a solution:
http://forum.unity3d.com/threads/ios-simulator.183097/
"Player settings > Other settings > SDK version > Simulator SDK"
as far as i remember it worked for me,
However this is the official unity answer (no) :
http://docs.unity3d.com/Manual/iphone-basic.html
Can Unity-built games run in the iPhone Simulator?
A: No, but Unity iOS can build to iPad Simulator if you’re using the latest SDK. However the simulator itself is not very useful for Unity because it does not simulate all inputs from iOS or properly emulate the performance you get on the iPhone/iPad. You should test out gameplay directly inside Unity using the iPhone/iPad as a remote control while it is running the Unity Remote application. Then, when you are ready to test performance and optimize the game, you publish to iOS devices.
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